Production of nursery material in Podkarpackie Region in the last years has shown an increasing tendency and in 2012 amounted to over 3.8 million of fruit trees seedlings. The issue of waste biomass from agricultural and nursery production is significant since each time there is a need of waste disposal. Not in each case it is possible to carry out in the form of natural fertilizer. However, the use for energy purposes is always possible. The objective of the paper was energy analysis of waste biomass from production of different types of fruit trees. Laboratory tests concerning calorific value of particular species of stocks for production of fruit trees and on the content of ash and elements (C, H, N and S) in their chemical composition were carried out. Assessment of biomass yield from 1 ha of the investigated types of stocks after tenoning was carried out.