The paper presents results of analysis of the size of mineral and natural fertilization in various cultivation technologies on NPK and humus in soil balance. The research was carried out for cultivation of plant species, which may be possibly used for production of bio-energy. Data was collected form operation sheet of the selected surveyed agricultural farms. 8 technologies of cultiva-tions, varied on account of total number of nitrogen, phosphorus and potassium doses in the applied mineral and natural fertilizers were investigated. With the use of the computer programme, developed within the scope and for the research need, NPK and soil humus balance were developed in relation to plants species and its nutrition demand, soil type and the amount of the obtained crop, was calcu-lated. From among the analysed cultivation technologies, the biggest losses in the amount of the researched elements (NPK) were reported for sugar beet cultivation (tech. 2W). They were respectively: nitrogen 157.00 kg·ha -1, phosphorus 93.00 kg·ha -1, potassium 385.00 kg·ha -1. Cultivation carried out in this technology depleted soil in humus by 1.54 t·ha -1. High, positive values of the balance were obtained for cultivation of rye for green forage (tech. 3M). Content of nitrogen in soil decreased by 202.60 kg·ha -1, phos-phorus by 235.20 kg·ha -1, potassium by 154.20 kg·ha-1. Calculations proved also in case of this technology, - increase in the amount of humus at the highest level by 4.38 t·ha -1. Based on results, one may state that in case of these technologies, doses and type of the applied fertilizers should be verified.