Directive 2009/128/EC on sustainable use of pesticides obligates the member states to include the technical condition of railway sprayers mounted on trains. Within the frame of the Multiannual Programme of the Research Institute of Horticulture a methodology for such inspection was elaborated. Basing on the literature review and on the own survey it was stated, that two kinds of the sprayers: sprayers similar to the field crop sprayers named here the “platform sprayers” and the rail-way units for chemical weed killing (Polish acronym – CHOT) are used for weed killing on the railways. The proposed methods and criteria for the inspection of elements of these sprayers do not considerably differ from the demands for the inspection of the field crop sprayers. The fundamental differences for the proposed methodology distinguishing it from the field crop sprayers, result from the place of application (railway track) and the driving speeds (up 40 km·h -1). Moreover, the new procedure of inspection of the injection systems used in the railway units for weed killing was suggested.